Developmental expression of GTP-binding proteins in rat testes
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چکیده
منابع مشابه
GTP-binding proteins in rat liver nuclear envelopes.
Nuclear transport as well as reassembly of the nuclear envelope (NE) after completion of mitosis are processes that have been shown to require GTP and ATP. To study the presence and localization of GTP-binding proteins in the NE, we have combined complementary techniques of [alpha-32P]GTP binding to Western-blotted proteins and UV crosslinking of [alpha-32P]GTP with well-established procedures ...
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Subfractionation of a canine pancreatic homogenate was performed by several differential centrifugation steps, which gave rise to fractions with distinct marker profiles. Specific binding of guanosine 5'-[gamma-[35S]thio]triphosphate (GTP[gamma-35S]) was assayed in each fraction. Enrichment of GTP[gamma-35S] binding was greatest in the interfacial "smooth" microsomal fraction, expected to conta...
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ژورنال
عنوان ژورنال: Reproduction
سال: 1993
ISSN: 1470-1626,1741-7899
DOI: 10.1530/jrf.0.0990487